製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to WDR33
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 32-153 of Human WDR33.

  • ポジティブ・コントロール
    • U 251 MG cells; Human colon tissue; RT 4, Human liver and tonsil lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性
    Most highly expressed in testis.
  • 配列類似性
    Contains 1 collagen-like domain.
    Contains 7 WD repeats.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • WD repeat containing protein 33 antibody
    • WD repeat domain 33 antibody
    • WD repeat-containing protein 33 antibody
    • WD repeat-containing protein WDC146 antibody
    • WDC146 antibody
    • WDR 33 antibody
    • WDR33 antibody
    • WDR33_HUMAN antibody
    see all

画像

  • All lanes : Anti-WDR33 antibody (ab121899) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab121899, at 1/45 dilution, staining WDR33 in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab121899, at 4 µg/ml, staining WDR33 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

参考文献

ab121899 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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