Anti-WDR12 抗体 (ab111955)
Key features and details
- Rabbit polyclonal to WDR12
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-WDR12 antibody
WDR12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR12 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-259 of Human WDR12 (AAH08082).
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ポジティブ・コントロール
- 293T, A431, Jurkat and Raji cells and whole cell lysates. Mouse brain lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111955の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
配列類似性
Belongs to the WD repeat WDR12/YTM1 family.
Contains 7 WD repeats. -
細胞内局在
Nucleus > nucleolus. Nucleus > nucleoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 55759 Human
- Entrez Gene: 57750 Mouse
- SwissProt: Q9GZL7 Human
- SwissProt: Q9JJA4 Mouse
- Unigene: 73291 Human
- Unigene: 281079 Mouse
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別名
- FLJ10881 antibody
- FLJ12719 antibody
- FLJ12720 antibody
see all
画像
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Anti-WDR12 antibody (ab111955) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Anti-WDR12 antibody (ab111955) at 1/1000 dilution + Mouse brain lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
ab111955 at 1/200 dilution staining WDR12 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab111955 は 2 報の論文で使用されています。
- Wang X et al. SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer. Nat Commun 12:2259 (2021). PubMed: 33859191
- Mi L et al. Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth. Front Oncol 11:751792 (2021). PubMed: 34868955