Anti-WDR11 抗体 (ab93871)
Key features and details
- Rabbit polyclonal to WDR11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WDR11 antibody
WDR11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR11 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to Human WDR11 aa 1150-1250.
Database link: Q9BZH6 -
ポジティブ・コントロール
- Whole cell lysate from HeLa and 293T cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 137 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
WDR11 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. The WDR11 gene is located in the chromosome 10q25-26 region, which is frequently deleted in gliomas and tumors of other tissues, and is disrupted by the t(10;19) translocation rearrangement in glioblastoma cells. The gene location suggests that it is a candidate gene for the tumor suppressor locus. -
細胞内局在
Membrane; Single-pass membrane protein, Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 55717 Human
- Omim: 606417 Human
- SwissProt: Q9BZH6 Human
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別名
- Bromodomain and WD repeat containing protein 2 antibody
- Bromodomain and WD repeat domain containing 2 antibody
- Bromodomain- and WD repeat-containing protein 2, formerly antibody
see all
画像
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All lanes : Anti-WDR11 antibody (ab93871) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 3 minutes -
ab93871 at 1 µg/ml detecting WDR11 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of WDR11
Lane 2: ab93871 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence an with exposure time of 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab93871 は 4 報の論文で使用されています。
- Yang B et al. Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. J Virol 96:e0182721 (2022). PubMed: 35020472
- Haag N et al. Biallelic loss-of-function variants in WDR11 are associated with microcephaly and intellectual disability. Eur J Hum Genet 29:1663-1668 (2021). PubMed: 34413497
- Navarro Negredo P et al. The WDR11 complex facilitates the tethering of AP-1-derived vesicles. Nat Commun 9:596 (2018). WB, ICC/IF . PubMed: 29426865
- Taylor KE & Mossman KL Cellular Protein WDR11 Interacts with Specific Herpes Simplex Virus Proteins at the trans-Golgi Network To Promote Virus Replication. J Virol 89:9841-52 (2015). PubMed: 26178983