製品の概要

  • 製品名Anti-WDHD1 antibody
    WDHD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to WDHD1
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residues 1079 and 1129 of human WDHD1.

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500. Use with methanol fixed cells.
WB 1/2000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
IP Use a concentration of 2 - 5 µg/ml.

ターゲット情報

  • 関連性The protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
  • 細胞内局在Nuclear; nucleoplasm.
  • 参照データベース
  • 別名
    • Acidic nucleoplasmic DNA binding protein 1 antibody
    • And 1 antibody
    • And1 antibody
    • WD repeat and HMG box DNA binding protein 1 antibody
    • WDHD 1 antibody
    see all

Anti-WDHD1 antibody 画像

  • Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72436 followed by WB (20% of IP loaded):
    Lane 1: ab72429 precipitate

    Lane 2: ab72436 precipitate

    Lane 3: Control IgG

    For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • All lanes : Anti-WDHD1 antibody (ab72436) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 126 kDa
    Observed band size : 126 kDa
  • ab72436 (1/500) staining WDHD1 in assynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

Anti-WDHD1 antibody (ab72436) 使用論文

This product has been referenced in:
  • Zhou Y  et al. Role of WDHD1 in Human Papillomavirus-Mediated Oncogenesis Identified by Transcriptional Profiling of E7-Expressing Cells. J Virol 90:6071-84 (2016). Read more (PubMed: 27099318) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

投稿 Mar 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"