製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to WDHD1
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Orangutan
  • 免疫原

    A synthetic peptide of a region between residues 975 and 1025 of human WDHD1.

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
  • 追加情報
    IP: Use at a concentration of 2 - 5 µg/mg lysate.
    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      The protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
    • 細胞内局在
      Nuclear; nucleoplasm.
    • 参照データベース
    • 別名
      • Acidic nucleoplasmic DNA binding protein 1 antibody
      • And 1 antibody
      • And1 antibody
      • WD repeat and HMG box DNA binding protein 1 antibody
      • WDHD 1 antibody
      see all

    画像

    • Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72429 followed by WB (20% of IP loaded):
      Lane 1: ab72429 precipitate

      Lane 2: ab72436 precipitate

      Lane 3: Control IgG

      For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.

      Detection: ReliaBLOT® Reagents and Procedures were incorporated followed by chemiluminescence with an exposure time of 10 seconds.

    プロトコール

    参考文献

    ab72429 has not yet been referenced specifically in any publications.

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