Anti-WDFY3 抗体 (ab84888)

製品の概要

  • 製品名Anti-WDFY3 antibody
  • 製品の詳細
    Rabbit polyclonal to WDFY3
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)

  • ポジティブ・コントロール
    • Human Controls: Breast Carcinoma, Ovarian Carcinoma, Placenta, Prostate Carcinoma

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified using an epitope specific to WDFY3 immobilized on solid support
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性WDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
  • 細胞内局在Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
  • 参照データベース
  • 別名
    • Alfy antibody
    • Autophagy linked FYVE protein antibody
    • KIAA0993 antibody
    • MGC16461 antibody
    • WD repeat and FYVE domain containing 3 antibody
    • ZFYVE25 antibody
    see all

Anti-WDFY3 antibody 画像

  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
  • ab84888 staining WDFY3 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.5% in PBS and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

Anti-WDFY3 antibody (ab84888) 使用論文

ab84888 has not yet been referenced specifically in any publications.

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To our knowledge, ab84888 has not been tested in WB. However by participating in our AbTrial program you can now use our products in an untested application or species without finan...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa (Cervical Epithelium Carcinoma))
Specification HeLa (Cervical Epithelium Carcinoma)
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"