製品の概要

  • 製品名Anti-WASP antibody
    WASP 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to WASP
  • 特異性This antibody reacts Wiskott-Aldrich Syndrome Protein. It is not expected to cross-react with N WASP (WASL).
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    C-SPADKKRSGKK KI

    , corresponding to Internal sequence amino acids 220-232 of Human WASP

  • ポジティブ・コントロール
    • U937 and MOLT4 cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab28769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Detection limit 1/128,000,
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 53 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
    • 組織特異性Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
    • 関連疾患Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
    • 配列類似性Contains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.
    • ドメインThe WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
    • 細胞内局在Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Anti-WASP antibody 画像

    • Anti-WASP antibody (ab28769) at 0.03 µg/ml + U937 cell lysate (total protein/per lane) at 30 µg/ml
      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 53-65 kDa (why is the actual band size different from the predicted?)

    Anti-WASP antibody (ab28769) 使用論文

    ab28769 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab28769.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"