製品の概要

  • 製品名Anti-WASH4P antibody
  • 製品の詳細
    Rabbit polyclonal to WASH4P
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human WASH4P aa 363-477.
    Sequence:

    GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQV RATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLP PPQQPQVDEDEDDWES


    Database link: A8MWX3

  • ポジティブ・コントロール
    • IHC-P: Human testis tissue WB: Human RT-4 and U-251 MG cell lysates

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting.
  • 参照データベース
  • 別名
    • FAM39CP antibody
    • Putative WAS protein family homolog 4 antibody

Anti-WASH4P antibody 画像

  • Immunohistochemical staining of WASH3P in Human testis tissue, using ab121151 at a 1/1000 dilution. The image shows strong nuclear positivity in cells of seminiferus ducts.
  • All lanes : Anti-WASH4P antibody (ab121151) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : A-431 cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles using ab121151.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

Anti-WASH4P antibody (ab121151) 使用論文

ab121151 has not yet been referenced specifically in any publications.

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