機能Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage.
配列類似性Contains 1 WW domain.
翻訳後修飾Phosphorylated on tyrosine residues.
細胞内局在Nucleus speckle. Nucleus. In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.
WW domain containing adapter protein with coiled coil antibody
WW domain containing adaptor with coiled coil antibody
WW domain-containing adapter protein with coiled-coil antibody
Anti-WAC antibody 画像
Western blot - WAC antibody (ab103994)
Anti-WAC antibody (ab103994) at 1/100 dilution + HepG2 cell lysate at 35 µg
Predicted band size : 71 kDa
Immunocytochemistry/ Immunofluorescence - WAC antibody (ab103994)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab103994 (1/200) staining WAC (KIAA1844) in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to abreview.