製品の概要

  • 製品名
    Anti-VSV-G tag antibody [P5D4]
    VSV-G tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [P5D4] to VSV-G tag
  • アプリケーション
    適用あり: ICC/IF, WB, IP, ICCmore details
  • 種交差性
    Reacts with VSV-G tag. Not yet tested in other species.
  • 免疫原

    Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH

  • ポジティブ・コントロール
    • Whole cell extracts expressing VSV-G tagged fusion protein.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940,  GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Proprietary Purification
  • 特記事項(精製)
    Purified from Tissue culture supernatant.
  • ポリ/モノ
    モノクローナル
  • クローン名
    P5D4
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21159872
WB Use a concentration of 0.5 - 1 µg/ml.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 別名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

参考文献

This product has been referenced in:
  • Malikov V  et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun 6:6660 (2015). WB . Read more (PubMed: 25818806) »
  • Lin AH  et al. Blockade of type I interferon (IFN) production by retroviral replicating vectors and reduced tumor cell responses to IFN likely contribute to tumor selectivity. J Virol 88:10066-77 (2014). Read more (PubMed: 24965455) »

See all 7 Publications for this product

レビューと Q&A

Thank you for contacting us.


Unfortunately we have not tested this antibody with an N-terminal VSVG tagged protein. The epitope for this clone (P5D4) resides within the C-terminal five amino acids (NRLGK) of the 15-amino acid antigenic ...

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Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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