製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VSV-G tag
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    Reacts with Vesicular stomatitis virus.
  • 免疫原

    Full length protein

  • 特記事項
    0.2 µ filtered

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.

ターゲット情報

  • 関連性
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 別名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody 画像

  • All lanes : Anti-VSV-G tag antibody (ab83196) at 1/1000 dilution

    Lane 1 : Human HEK293 cells transfected with pMD2.G (VSVG encoding construct) whole cell lysate (labelled +VSVG)
    Lane 2 : Human HEK293 mock transfected (labelled -VSVG)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa


    Exposure time : 10 seconds

    Image is courtesy of an anonymous AbReview.

    See Abreview

プロトコール

Anti-VSV-G tag antibody (ab83196) 使用論文

ab83196 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Treatment
Transfected with pMD2.G or mock
Specification
HEK293 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
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投稿 Sep 28 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293 cell line)
Permeabilization
No
Specification
HEK293 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 Sep 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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