The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
Vesicular stomatitis virus glycoprotein tag antibody
VSV epitope tag antibody
VSV tag antibody
VSV-G epitope tag antibody
vsvg tag antibody
Western blot - Anti-VSV-G tag antibody (ab83196)Image is courtesy of an anonymous AbReview.
All lanes : Anti-VSV-G tag antibody (ab83196) at 1/1000 dilution
Lane 1 : Human HEK293 cells transfected with pMD2.G (VSVG encoding construct) whole cell lysate (labelled +VSVG) Lane 2 : Human HEK293 mock transfected (labelled -VSVG)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution