製品の概要

  • 製品名Anti-VSV-G tag antibody
    VSV-G tag 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VSV-G tag
  • 特異性This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
  • アプリケーション適用あり: ICC, IHC-Fr, WB, IP, ICC/IFmore details
  • 免疫原

    A synthetic peptide corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence:

    YTDIEMNRLGK

    .

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 15662032
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF 1/200.

ターゲット情報

  • 関連性Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 別名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody 画像

  • ab1874 detecting bound HS scFv antibody in rat lung tissue by Immunohistochemistry (Frozen sections).
    Lungs from Sprague-Dawley rats were harvested at embryonic days 17.5 of gestation and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4. The lungs were washed in PBS, cryoprotected with 20% (w/v) sucrose in PBS, and gelatin-embedded (7.5% (w/v) gelatin, 15% (w/v) sucrose in PBS) before being covered in Cryo-M-Bed and snap frozen at -40 °C. The lung sections were cut at 8 µm and mounted onto chrome alum gel slides for storage at -40 °C. Tissue sections were blocked with 10% (v/v) goat serum in PBS and incubated overnight at 4 °C with HS scFv antibody, diluted 1/5 in 1% (v/v) goat serum in PBS. Bound antibody was detected with ab1874 diluted 1/200 in 1% (v/v) goat serum in PBS, followed by fluorescein isothiocyanate-conjugated goat anti-rabbit IgG.

    aw, airway; me, mesenchyme; bm, basement membrane.

Anti-VSV-G tag antibody (ab1874) 使用論文

This product has been referenced in:
  • Havekes R  et al. Compartmentalized PDE4A5 Signaling Impairs Hippocampal Synaptic Plasticity and Long-Term Memory. J Neurosci 36:8936-46 (2016). WB, IHC, ICC/IF . Read more (PubMed: 27559174) »
  • Niemelä EH  et al. Global analysis of the nuclear processing of transcripts with unspliced U12-type introns by the exosome. Nucleic Acids Res 42:7358-69 (2014). Read more (PubMed: 24848017) »

See all 9 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Saccharomyces cerevisiae Cell
Fixative Formaldehyde
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Username

Mr. Chesneau Laurent

Verified customer

投稿 Sep 19 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Mr. Chesneau Laurent

Verified customer

投稿 Sep 02 2005

The antibody has not been tested in this way so we do not know it it will bind.

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody ...

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Thank you for contacting us and your interest in our products.

As discussed over the phone, I have looked into the antibodies we have which are directed against the VSV-G tag. As you have seen, the reference where ab1874 was used in the stain...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"