Anti-VSIG4 抗体 (ab56037)

製品の概要

  • 製品名Anti-VSIG4 antibody
    VSIG4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VSIG4
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: SHYTCEVTWQ TPDGNQVVRD KITELRVQKL SVSKPTVTTG SGYGFTVPQG, corresponding to N terminal amino acids 109-158 of Human VSIG4

  • ポジティブ・コントロール
    • Jurkat cell lyaste.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab56037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
  • 組織特異性Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.
  • 配列類似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Complement receptor of the immunoglobulin superfamily antibody
    • CRIg antibody
    • Ig superfamily protein antibody
    • Protein Z39Ig antibody
    • UNQ317/PRO362 antibody
    • V set and immunoglobulin domain containing 4 antibody
    • V-set and immunoglobulin domain-containing protein 4 antibody
    • VSIG4 antibody
    • VSIG4_HUMAN antibody
    • Z39IG antibody
    see all

Anti-VSIG4 antibody 画像

  • Anti-VSIG4 antibody (ab56037) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-VSIG4 antibody (ab56037) 使用論文

ab56037 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (monocytes from PBMC, Jurkat, THP-1, HEK293)
Permeabilization No
Gating Strategy CD14+ monocytes from PBMC respective cell line (see above)
Specification monocytes from PBMC, Jurkat, THP-1, HEK293
Preparation Cell harvesting/tissue preparation method: Monocytes were stained within PBMC which was isolated by density gradient centrifugation of whole blood Jurkat, THP-1 and HEK293 cell line were harvested out of cell culture flasks and resuspended in FACS Azide buffer
Sample buffer: FACS Azide buffer was used consisting of PBS + 2 mM EDTA + 0,1% Natriumazid + 2% FCS
Fixation no fixation
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投稿 Apr 13 2015

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