製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VR1
  • アプリケーション
    適用あり: IHC (PFA fixed), IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Guinea pig
  • 免疫原

    Synthetic peptide:

    (C)EDAEVFKDSMAPGEK

    conjugated to KLH, corresponding to C terminal amino acids 825 - 839 of Mouse TRPV1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC (PFA fixed) 1/1000.
IHC-P 1/1000.
IHC-Fr 1/400. PubMed: 19153713

ターゲット情報

  • 機能
    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • 組織特異性
    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • 配列類似性
    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • ドメイン
    The association domain (AD) is necessary for self-association.
  • 翻訳後修飾
    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • 細胞内局在
    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Anti-VR1 antibody 画像

  • ab31895 at 1/1000 demonstrating VR1 immunoreactivity in mouse dorsal root ganglion. Animals were perfusion fixed with 2% paraformaldehyde.
  • ab31895 staining mouse inferior olive using Cy3-conjugated donkey anti-rabbit secondary antibodies (red color) and DAPI (blue) as a nuclear counterstain.

プロトコール

Anti-VR1 antibody (ab31895) 使用論文

This product has been referenced in:
  • Koh WU  et al. The preventive effect of resiniferatoxin on the development of cold hypersensitivity induced by spinal nerve ligation: involvement of TRPM8. BMC Neurosci 17:38 (2016). WB . Read more (PubMed: 27329106) »
  • Chen Y  et al. Possible Loss of GABAergic Inhibition in Mice With Induced Adenomyosis and Treatment With Epigallocatechin-3-Gallate Attenuates the Loss With Improved Hyperalgesia. Reprod Sci N/A:N/A (2014). Mouse . Read more (PubMed: 24492488) »

See all 13 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (olfactory epithelium)
Loading amount
40 µg
Specification
olfactory epithelium
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2011

Thank you for contacting us. I understand your customer's concerns regarding the anti-VR1 antibody ab31895. Please be assured that the observed precipitation sometimes happens for whole antiserum antibodies and there is no problem with t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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