製品の概要

  • 製品名Anti-VPS4a antibody
    VPS4a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VPS4a
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (VEYFLHAIKY EAHSDKAKES IRAKCVQYLD RAEKLKDYLR SKEKHGKKPV) of human VPS4a (NP_037377).

  • ポジティブ・コントロール
    • Fetal muscle lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the AAA ATPase family.
    Contains 1 MIT domain.
  • ドメインThe MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
  • 細胞内局在Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hVPS4 antibody
    • Protein SKD2 antibody
    • SKD1 antibody
    • SKD1 homolog antibody
    • SKD1A antibody
    • SKD2 antibody
    • Vacuolar protein sorting 4 homolog A antibody
    • Vacuolar protein sorting 4, yeast, homolog of, A antibody
    • Vacuolar protein sorting factor 4A antibody
    • Vacuolar protein sorting-associated protein 4A antibody
    • vacuolar sorting protein 4 antibody
    • VPS4 antibody
    • VPS4-1 antibody
    • Vps4a antibody
    • VPS4A_HUMAN antibody
    see all

Anti-VPS4a antibody 画像

  • Anti-VPS4a antibody (ab81697) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa

Anti-VPS4a antibody (ab81697) 使用論文

This product has been referenced in:
  • Bleck M  et al. Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding. Proc Natl Acad Sci U S A 111:12211-6 (2014). WB . Read more (PubMed: 25099357) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab81697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"