製品の概要

  • 製品名
    Anti-VPS39 antibody
    VPS39 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to VPS39
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human VPS39 protein (NP_056104.2)

  • ポジティブ･コントロール
    • VPS39 transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 102 kDa).

ターゲット情報

  • 機能
    May play a role in clustering and fusion of late endosomes and lysosomes.
  • 組織特異性
    Ubiquitous. Highly expressed in heart, skeletal muscle, kidney, pancreas, brain, placenta and lung.
  • 配列類似性
    Belongs to the VAM6/VPS39 family.
    Contains 1 CNH domain.
  • 細胞内局在
    Cytoplasm. Lysosome membrane. Late endosome membrane.
  • Information by UniProt
  • 参照データベース
    • 製品の状態
      There are 2 isoforms produced by alternative splicing.
    • 別名
      • A230065P22Rik antibody
      • AW743070 antibody
      • FLJ21681 antibody
      • FLJ46546 antibody
      • hVam6p antibody
      • KIAA0770 antibody
      • RP23-348L16.12 antibody
      • TLP antibody
      • TRAP1 like protein antibody
      • Vacuolar protein sorting 39 antibody
      • Vacuolar protein sorting 39 homolog antibody
      • VAM6 antibody
      • Vam6/Vps39 like antibody
      • Vam6/Vps39 like protein antibody
      • Vam6/Vps39-like protein antibody
      • VPS 39 antibody
      • Vps39 antibody
      • VPS39_HUMAN antibody
      see all

    画像

    • Western blot - VPS39 antibody (ab69669)
      Western blot - VPS39 antibody (ab69669)
      All lanes : Anti-VPS39 antibody (ab69669) at 1/500 dilution

      Lane 1 : VPS39 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

      Predicted band size: 102 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    This product has been referenced in:
    • Liu X  et al. Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus. J Biol Chem 286:17079-90 (2011). Read more (PubMed: 21454559) »

    See 1 Publication for this product

    ab69669 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    Western blot abreview for Anti-VPS39 antibody

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hela, 293)
    Loading amount
    5 µg
    Specification
    hela, 293
    Gel Running Conditions
    Non-reduced Denaturing (10% SDS running gel, 150V)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    投稿 Jul 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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