The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.
Widely expressed. Expressed in macrophages and lymphocytes.
Belongs to the VPS37 family. Contains 1 VPS37 C-terminal domain.
Late endosome membrane. Recruited to the endosomal membrane in a VPS4A-dependent fashion.