製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VPS35
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 733-796 (KENDAVTIQV LNQLIQKIRE DLPNLESSEE TEQINKHFHN TLEHLRLRRE SPESEGPIYE GLIL) of Human VPS35 (NP_060676).

  • ポジティブ・コントロール
    • H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.

ターゲット情報

  • 機能
    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • 組織特異性
    Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
  • 配列類似性
    Belongs to the VPS35 family.
  • 細胞内局在
    Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp434E1211 antibody
    • DKFZp434P1672 antibody
    • FLJ10752 antibody
    • FLJ13588 antibody
    • FLJ20388 antibody
    • hVPS35 antibody
    • Maternal embryonic 3 antibody
    • Maternal-embryonic 3 antibody
    • MEM 3 antibody
    • MEM3 antibody
    • PARK17 antibody
    • TCCCTA00141 antibody
    • Vacuolar protein sorting 35 (yeast) antibody
    • Vacuolar protein sorting 35 antibody
    • Vacuolar protein sorting 35 homolog antibody
    • Vacuolar protein sorting associated protein 35 antibody
    • Vacuolar protein sorting-associated protein 35 antibody
    • Vesicle protein sorting 35 antibody
    • VPS 35 antibody
    • VPS35 antibody
    • VPS35_HUMAN antibody
    see all

Anti-VPS35 antibody 画像

  • All lanes : Anti-VPS35 antibody (ab97545) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : MOLT4 whole cell lysate
    Lane 5 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 92 kDa

プロトコール

Anti-VPS35 antibody (ab97545) 使用論文

This product has been referenced in:
  • Zhou M  et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. J Cell Biol 214:445-58 (2016). Read more (PubMed: 27502489) »
  • McGough IJ  et al. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling. J Cell Sci 127:4940-53 (2014). Read more (PubMed: 25278552) »

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (mouse cortical primary neuron culture DIV14)
Permeabilization
Yes - 0.2% Triton X-100
Specification
mouse cortical primary neuron culture DIV14
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Julie Nys

Verified customer

投稿 Nov 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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