製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to VPS29
  • 特異性
    This antibody is expected to recognise both reported isoforms
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Dog, Human
  • 免疫原

    Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence

  • ポジティブ・コントロール
    • Human spleen lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 8 µg/ml. In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands.
WB Use at an assay dependent dilution. Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3µg/ml antibody staining (expected size : 20.9kDa). The 60kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size but we would welcome any feedback.

ターゲット情報

  • 機能
    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).
  • 組織特異性
    Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
  • 配列類似性
    Belongs to the VPS29 family.
  • 細胞内局在
    Cytoplasm. Membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DC15 antibody
    • DC7 antibody
    • DKFZp564F0223 antibody
    • FLJ20492 antibody
    • hVPS29 antibody
    • PEP11 antibody
    • PEP11 homolog antibody
    • Retromer protein antibody
    • Vacuolar protein sorting 29 antibody
    • Vacuolar protein sorting 29 (yeast homolog) antibody
    • Vacuolar protein sorting 29 (yeast) antibody
    • Vacuolar protein sorting 29 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 29 homolog antibody
    • Vacuolar protein sorting associated protein 29 antibody
    • Vacuolar protein sorting-associated protein 29 antibody
    • Vacuolar sorting protein VPS29/PEP11 antibody
    • Vesicle protein sorting 29 antibody
    • VPS 29 antibody
    • VPS29 antibody
    • VPS29 retromer complex component antibody
    • VPS29_HUMAN antibody
    • x 007 protein antibody
    see all

Anti-VPS29 antibody 画像

  • ab51972 at 3.8 µg/ml staining VPS29 in Human uterus tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody detected by AP-staining.

Anti-VPS29 antibody (ab51972) 使用論文

ab51972 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab51972.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録