製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to VPS28
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide: ESAYNAFNRFLHA, corresponding to C terminal amino acids 209-211 of Human VPS28.

  • ポジティブ・コントロール
    • Human testis and brain lysates.
  • 特記事項


    This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考
    This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).Can be blocked with Human VPS28 peptide (ab23183).

ターゲット情報

  • 機能
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
  • 配列類似性
    Belongs to the VPS28 family.
    Contains 1 VPS28 C-terminal domain.
    Contains 1 VPS28 N-terminal domain.
  • 細胞内局在
    Cell membrane. Late endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ESCRT-I complex subunit VPS28 antibody
    • H-Vps28 antibody
    • Vacuolar protein sorting 28 antibody
    • Vacuolar protein sorting 28 (yeast) antibody
    • Vacuolar protein sorting 28 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 28 homolog antibody
    • Vacuolar protein sorting-associated protein 28 homolog antibody
    • VPS 28 antibody
    • Vps28 antibody
    • VPS28_HUMAN antibody
    • Yeast class E protein Vps28p homolog antibody
    see all

画像



  • Predicted band size : 25 kDa


    ab10133 at 1µg/ml staining approximately 24kDa band of human VPS28 in human testis lysate by Western blot (ECL).
  • ab10133 (10µg/ml) staining of Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer pH9 and HRP-staining.

プロトコール

参考文献

This product has been referenced in:
  • Mikula M  et al. Genome-wide co-localization of active EGFR and downstream ERK pathway kinases mirrors mitogen-inducible RNA polymerase 2 genomic occupancy. Nucleic Acids Res 44:10150-10164 (2016). Read more (PubMed: 27587583) »

See 1 Publication for this product

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