製品の概要

  • 製品名Anti-VPS25 antibody
    VPS25 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to VPS25
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Dog
  • 免疫原

    Synthetic peptide corresponding to Human VPS25 aa 20-29 (internal sequence).
    Sequence:

    C-QPNVDTRQKQ

  • ポジティブ・コントロール
    • WB: Human liver and rat kidney tissue lysates. IHC-P: Human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab42500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 21 kDa.

ターゲット情報

  • 機能Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
  • 組織特異性Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.
  • 配列類似性Belongs to the VPS25 family.
  • 細胞内局在Cytoplasm. Endosome membrane. Nucleus > nucleoplasm. Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dermal papilla derived protein 9 antibody
    • Dermal papilla-derived protein 9 antibody
    • DERP9 antibody
    • EAP20 antibody
    • ELL associated protein of 20 kDa antibody
    • ELL-associated protein of 20 kDa antibody
    • ESCRT-II complex subunit VPS25 antibody
    • FAP20 antibody
    • HGNC:28122 antibody
    • hVps25 antibody
    • MGC10540 antibody
    • Vacuolar protein sorting 25 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 25 antibody
    • Vacuolar protein-sorting-associated protein 25 antibody
    • VPS25 antibody
    • VPS25_HUMAN antibody
    see all

Anti-VPS25 antibody 画像

  • Anti-VPS25 antibody (ab42500) at 0.5 µg/ml + Rat kidney tissue lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa

    Incubated with the primary antibody for 1 hour.

  • ab42500 (10µg/ml) staining human kidney by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Anti-VPS25 antibody (ab42500) at 0.5 µg/ml + Human liver tissue lysate (in RIPA buffer). at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

Anti-VPS25 antibody (ab42500) 使用論文

ab42500 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"