製品の概要

  • 製品名Anti-VPS24 antibody
    VPS24 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VPS24
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: RVVDRQIRDI QREEEKVKRS VKDAAKKGQK DVCIVLAKEM IRSRKAVSKL, corresponding to amino acids 28-77 of Human VPS24

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor.
  • 組織特異性Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 配列類似性Belongs to the SNF7 family.
  • ドメインThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • 細胞内局在Cytoplasm > cytosol. Membrane. Endosome. Late endosome membrane. Localizes to the midbody of dividing cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • VPS 24 antibody
    • VPS24 antibody
    • CGI 149 antibody
    • CGI149 protein antibody
    • Charged multivesicular body protein 3 antibody
    • CHMP3 antibody
    • CHMP3_HUMAN antibody
    • Chromatin modifying protein 3 antibody
    • Chromatin-modifying protein 3 antibody
    • HGNC:29865 antibody
    • hVps24 antibody
    • NEDF antibody
    • Neuroendocrine differentiation factor antibody
    • Vacuolar protein sorting 24 (yeast) antibody
    • Vacuolar protein sorting 24 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 24 antibody
    • Vacuolar protein sorting-associated protein 24 antibody
    • vps24 antibody
    see all

Anti-VPS24 antibody 画像

  • Anti-VPS24 antibody (ab49266) at 0.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • IHC image of ab49266 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49266, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-VPS24 antibody (ab49266) 使用論文

ab49266 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 2 µg
Gel Running Conditions Reduced Denaturing (4-12% Gel)
Sample Rat Cell lysate - whole cell (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Aug 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"