Anti-VPRBP 抗体 (ab75458)

Anti-VPRBP antibody 画像

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  Western blot - VPRBP antibody (ab75458)
  All lanes : Anti-VPRBP antibody (ab75458) at 1 µg/ml
  
  Lane 1 : Liver (Rat) Tissue Lysate
  Lane 2 : : Liver (Mouse) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 169 kDa
  Observed band size : 169 kDa
  Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
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  Immunocytochemistry/ Immunofluorescence - VPRBP antibody (ab75458)
  ICC/IF image of ab75458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml.
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPRBP antibody (ab75458)
  IHC image of VPRBP staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75458 at 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.