製品の概要
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製品名Anti-VPRBP antibody
VPRBP 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to VPRBP
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アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Horse, Cow, Dog
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human VPRBP.
(Peptide available as ab86242.)
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ポジティブ･コントロール
- This antibody gave a positive signal in Rat and Mouse Liver Tissue Lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab75458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 169 kDa (predicted molecular weight: 169 kDa). |
ターゲット情報
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関連性VprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase.
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細胞内局在Cytoplasmic and Nuclear
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参照データベース
- Entrez Gene: 9730 Human
- Entrez Gene: 321006 Mouse
- Entrez Gene: 315987 Rat
- SwissProt: Q9Y4B6 Human
- SwissProt: Q80TR8 Mouse
- Unigene: 716623 Human
- Unigene: 440089 Mouse
- Unigene: 32961 Rat
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別名
- DCAF1 antibody
- DDB1 and CUL4 associated factor 1 antibody
- HIV-1 Vpr-binding protein antibody
see all
Anti-VPRBP antibody 画像
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Western blot - VPRBP antibody (ab75458)All lanes : Anti-VPRBP antibody (ab75458) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 169 kDa
Observed band size : 169 kDa
Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - VPRBP antibody (ab75458)ICC/IF image of ab75458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPRBP antibody (ab75458)IHC image of VPRBP staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75458 at 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
Anti-VPRBP antibody (ab75458) 使用論文
This product has been referenced in:
- Cassiday PA et al. Understanding the molecular manipulation of DCAF1 by the lentiviral accessory proteins Vpr and Vpx. Virology 476:19-25 (2015). Read more (PubMed: 25499532) »
- Kogan M et al. Inhibition of NF-?B activity by HIV-1 Vpr is dependent on Vpr binding protein. J Cell Physiol : (2012). WB ; Human . Read more (PubMed: 23001849) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"