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Synthetic peptide derived from within the activation domain (amino acids 413-490) of HSV VP16.
Our Abpromise guarantee covers the use of ab4808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 30 kDa.Can be blocked with VP16 tag peptide (ab13406).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488 ® conjugated Donkey anti-Rabbit IgG.
This image is courtesy of an Abreview submitted on 10 March 2006.