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Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.
Our Abpromise guarantee covers the use of ab6994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||1/200. (see Abreview)|
|IHC-P||1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.|
|Flow Cyt||Use a concentration of 5 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19622235|
Confocal extended focus photograph of von Willebrand factor staining (red) and non-specific nuclear counterstain (green) in the adult mouse olfactory bulb. This picture shows a single major vessel within the glomerular layer of the olfactory bulb. For more details see the review of this antibody by Adam Puche.
Immunocytochemical analysis labeling Von Willebrand Factor with ab6994 at 1/100 dilution.
The nuclear counterstain is DAPI(blue)
ab6994 staining Von Willebrand Factor in pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was enzymatic using pronase, 1mg/ml. Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"