製品の概要

  • 製品名
    Anti-Von Willebrand Factor antibody
    Von Willebrand Factor 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Von Willebrand Factor
  • アプリケーション
    適用あり: IHC-Fr, Immunodiffusion, Flow Cyt, ELISA, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human, Pig
  • 免疫原

    Purified human von Willebrand factor.

  • ポジティブ・コントロール
    • Tonsil.
  • 特記事項
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50 - 1/100.
Immunodiffusion 1/1.
Flow Cyt Use at an assay dependent concentration.

ab37385 - Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
    • 組織特異性
      Plasma.
    • 関連疾患
      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
    • 配列類似性
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.
    • ドメイン
      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
    • 翻訳後修飾
      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.
    • 細胞内局在
      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    画像

    • ab11713 at a 1/1000 dilution staining Von Willebrand Factor in mouse blood vessels by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at 19°C. Secondary used at 1/200 polyclonal Donkey anti-sheep conjugated to Alexa Fluor 647 (pink). Counterstain (blue) DAPI.

      See Abreview

    • ab11713 staining Von Willebrand Factor in porcine heart tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked with 10% serum for 30 minutes at 22°C and then incubated with ab11713 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated donkey anti-sheep polyclonal used at a 1/1000 dilution.

      See Abreview

    参考文献

    This product has been referenced in:
    • Yurdagul A  et al. Oxidized LDL induces FAK-dependent RSK signaling to drive NF-?B activation and VCAM-1 expression. J Cell Sci 129:1580-91 (2016). Read more (PubMed: 26906414) »
    • Claude C  et al. Inhalable delivery of AAV-based MRP4/ABCC4 silencing RNA prevents monocrotaline-induced pulmonary hypertension. Mol Ther Methods Clin Dev 2:14065 (2015). Read more (PubMed: 26052533) »

    See all 18 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Keloid)
    Permeabilization
    No
    Specification
    Keloid
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Sep 15 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung)
    Permeabilization
    Yes - Triton 0.1%
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Zinc fixative
    Username

    Abcam user community

    Verified customer

    投稿 Jul 25 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (A7r5 smooth muscle cells)
    Permeabilization
    Yes - Triton and Tween
    Specification
    A7r5 smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Username

    Julie Kohn

    Verified customer

    投稿 May 02 2016

    Application
    Immunocytochemistry
    Sample
    Guinea pig Cell (Endothelilal cells freshly isolated)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Endothelilal cells freshly isolated
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Sep 23 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skin)
    Gel Running Conditions
    Reduced Denaturing (7.5%gel)
    Loading amount
    125 µg
    Treatment
    Wound
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 27 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (brain, liver)
    Specification
    brain, liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 05 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (skin dermis)
    Specification
    skin dermis
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    投稿 May 30 2014

    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Sample
    Mouse Cell (skin)
    Specification
    skin
    Preparation
    Cell harvesting/tissue preparation method: Wilson L, Fathke C, Isik F. Tissue dispersion and flow cytometry for the cellular analysis of wound healing. BioTechniques 2002: 32: 548-551.
    Sample buffer: 2%BSA/PBS
    Username

    Abcam user community

    Verified customer

    投稿 May 14 2014

    Thank you for contacting Abcam.

    I ran an alignment of the human and chicken VWF protein sequence, and there is only 56.1% homology between the two. Our AbTrial program, which allows researchers to test our products in untested species and rece...

    Read More

    Thank you very much for your call today and for your patience while I've been in touch with the production lab regarding your enquiry.
    The IgG concentration of this lot is 9.13 mg/ml.
    I hope that this information will be useful, but if you ha...

    Read More

    1-10 of 25 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録