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Our Abpromise guarantee covers the use of ab20435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22280443|
|Flow Cyt||Use 2µg for 105 cells. . ab20435 has been used in flow cytometry with Jurkat cells and with platelets. The Jurkat cell line was fixed using 1% formaldehyde followed by permeabilization using a commercially-available saponin reagent for permeabilization. The platelets were fixed using 1% formaldehyde, washed and suspended in PBS without permeabilization.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/50 - 1/100.|