Anti-Von Willebrand Factor 抗体 [2Q2134] (ab68545)

製品の概要

  • 製品名Anti-Von Willebrand Factor antibody [2Q2134]
    Von Willebrand Factor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2Q2134] to Von Willebrand Factor
  • 特異性ab68545 reacts strongly with human von Willebrand Factor in both plasma and tissues.
  • アプリケーション適用あり: IP, IHC-P, RIA, IHC-Fr, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    Full length protein (Human)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. For Indirect Immunoperoxidase staining: Use at 1/50 dilution.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 組織特異性Plasma.
  • 関連疾患Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • 配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • ドメインThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • 翻訳後修飾All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • 細胞内局在Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody [2Q2134] (ab68545) 使用論文

This product has been referenced in:
  • Balaoing LR  et al. Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. PLoS One 10:e0130749 (2015). IF ; Human . Read more (PubMed: 26090873) »
  • Chen D  et al. Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy. Int J Mol Med 36:1563-71 (2015). Read more (PubMed: 26459716) »

See all 6 Publications for this product

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There is very little epitope information about any of the Von Willebrand Factor antibodies; because of this few have been identified as recognizing the N-terminus of vWF. Some of them may recognize the N-term,...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich bezüglich des Von Willebrand FactorAntikörpers ab11713 im Labor nachgefragt. Ich kann Ihnen nun bestätigen, dass dieser Antikörper spezifisch für den Von Will...

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Thank you for your call today and for your questions about our VWF antibodies.
As we discussed, ab68545 would be a good antibody to try in sandwich ELISA with ab6994. I have issued a discount code that is worth 1 free primary antibody after submit...

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Thank you for getting back to me and for gathering more information about the customer's assay.

I have conducted some search for your customer and found some products which might be suitable for this application. However, it is important to e...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (lung tissue)
Specification lung tissue
Fixative Formaldehyde
Permeabilization No
Blocking step No blocking step used for 1 hour(s) and 0 minute(s)
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投稿 Jun 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"