製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VMAT1
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 476-525 (YYLRSPPAKEEKLAILSQDCPMETRMYATQKPTKEFPLGEDSDEEPDHE E) of Human VMAT1, NP_003044

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

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  • Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections) analysis of human brain cortex tissue labeling VMAT1 with ab86325.

  • Anti-VMAT1 antibody (ab86325) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Gel concentration: 12%
    We are unsure as to the identity of the extra band.
  • Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections) analysis of human brain cortex tissue labeling VMAT1 with ab86325 at 2.5ug/ml. HRP/DAB detection.

参考文献

ab86325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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