製品の概要

  • 製品名Anti-VLDL Receptor antibody
    VLDL Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VLDL Receptor
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human VLDL Receptor.

    (Peptide available as ab102057.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human ovary tissue lysate.

アプリケーション

Our Abpromise guarantee covers the use of ab92943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 96 kDa).

ターゲット情報

  • 機能Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.
  • 組織特異性Abundant in heart and skeletal muscle; also ovary and kidney; not in liver.
  • 関連疾患Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures.
  • 配列類似性Contains 3 EGF-like domains.
    Contains 8 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • 翻訳後修飾Ubiquitinated at Lys-839 by MYLIP leading to degradation.
  • 細胞内局在Membrane. Membrane > clathrin-coated pit.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ35024 antibody
    • Very low density lipoprotein receptor antibody
    • Very low-density lipoprotein receptor antibody
    • VLDL R antibody
    • VLDL receptor antibody
    • VLDL-R antibody
    • VLDLR antibody
    • VLDLR_HUMAN antibody
    • VLDLRCH antibody
    see all

Anti-VLDL Receptor antibody 画像

  • Anti-VLDL Receptor antibody (ab92943) at 1 µg/ml + Human ovary tissue lysate - total protein (ab30222) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 96 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa,63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

Anti-VLDL Receptor antibody (ab92943) 使用論文

This product has been referenced in:
  • Yang XP  et al. Scavenger receptor-BI is a receptor for lipoprotein(a). J Lipid Res 54:2450-7 (2013). Read more (PubMed: 23812625) »
  • Chen X  et al. Up-regulation of ATP Binding Cassette Transporter A1 Expression by Very Low Density Lipoprotein Receptor and Apolipoprotein E Receptor 2. J Biol Chem 287:3751-9 (2012). WB ; Mouse . Read more (PubMed: 22170052) »

See all 2 Publications for this product

Product Wall

From your description it sounds like you are seeing something like what we show on the datasheet, a band at 130 kDa that we think is the receptor, and cross-reactivity with smaller proteins. The "predicted" size of 96 kDa is just that, a prediction bas...

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Regarding a western blot protocol modification for the VLDLr antibody ab92943, this antibody does not require anything unusual, and I expect your protocol is fine if it is working for other antibodies. However, I recommend having a look at a recent cus...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney, Testes, Cerebellum and spleen (see figure))
Loading amount 15 µg
Specification Kidney, Testes, Cerebellum and spleen (see figure)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel (Novex))
Blocking step Milk, serum (see figure) and BSA tested as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Oct 30 2012

I am very pleased to hear you would like to accept our offer and test ab92943 in mouse samples.

DISCOUNT CODE: 100%ABR-EB3EY
Expiration date: 1st Feb 2012

This code will give you: 1 free primary antibody before the expiration...

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Thank you very much for yourenquiry.

I am sorry to confirm thatour knowledge, ab92943 has not been tested in mouse. It is predicted to react, due to high sequence alignment of the immmunogen with the mouse VLDL Receptor sequence. However, I ...

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Thank you for contacting us.


As we had spoken of I have instructed our products team to add the blocking peptide for this ab92943. The product number is ab102057 and can be found at the following link: http://www.abcam.com/VLDL-Receptor-...

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Thank you for your enquiry.

This product has only been tested withHuman samples usingwestern blot (WB). The cross reactivity with rat is solely based on homology sequence (94% homology) with the immunogen.Therefore, I am afraid that we will ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"