1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K.
Expressed at highest levels in fetal and adult liver, followed by fetal heart, kidney, and lung, adult heart, and pancreas.
Defects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2) [MIM:607473]. VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K. Defects in VKORC1 are a cause of coumarin resistance (CMRES) [MIM:122700]; also known as warfarin resistance. Warfarin and other coumarin-type anticoagulants are used to reduce blood viscosity in the treatment of thromboembolic disorders.