The monoclonal antibody ND-3G6 binds with high affinity to vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica). The antibody also cross-reacts with vitellogenin from other bird species, including American kestrel (Falco sparverius), zebra finch (Taeniopygia guttata) and domestic chicken (Gallus gallus). The degree of cross-reactivity differs between species and with the methods employed.
Mixture of Vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/100 - 1/1000.
WB: 1/50 - 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitellogenin antibody [ND-3G6] (ab36794)Image courtesy of Dr Peter Wooding by Abreview.
ab36794 staining Vitellogenin in chicken ovary sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde, blocked using 1% BSA for 30 minutes at 20°C, then incubated with ab36794 at a 1/20 dilution for 20 hours at 4°C. The secondary used was a 5nm Gold conjugated goat anti-rabbit polyclonal used at a 1/40 dilution. Counterstained with HandE.