Anti-Vitellogenin 抗体 [ND-3G6] (ab36794)

製品の概要

  • 製品名
    Anti-Vitellogenin antibody [ND-3G6]
    Vitellogenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ND-3G6] to Vitellogenin
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    The monoclonal antibody ND-3G6 binds with high affinity to vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica). The antibody also cross-reacts with vitellogenin from other bird species, including American kestrel (Falco sparverius), zebra finch (Taeniopygia guttata) and domestic chicken (Gallus gallus). The degree of cross-reactivity differs between species and with the methods employed.
  • 免疫原

    Mixture of Vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: BSA
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    ND-3G6
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
ELISA
WB
  • 追加情報
    ELISA: 1/100 - 1/1000.
    WB: 1/50 - 1/500.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • 細胞内局在
      Secreted
    • 別名
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    画像

    • Coating: Purified Vtg (2 μg/ml) or egg yolk (y; diluted 1:500). Primary antibody: ND-3G6
    • ab36794 staining Vitellogenin in chicken ovary sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in formaldehyde, blocked using 1% BSA for 30 minutes at 20°C, then incubated with ab36794 at a 1/20 dilution for 20 hours at 4°C. The secondary used was a 5nm Gold conjugated goat anti-rabbit polyclonal used at a 1/40 dilution. Counterstained with HandE.

      See Abreview

    参考文献

    ab36794 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (ovaries)
    Specification
    ovaries
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Dr. Peter Wooding

    Verified customer

    投稿 Sep 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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