The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/100 - 1/1000.
Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
Western blot - Anti-Vitellogenin [JE-2A6] antibody (ab36805)
All lanes : Anti-Vitellogenin antibody [JE-2A6] (ab36805) at 1/200 dilution
Lane 1 : Purified zebrafish vitellogenin at 2 µg Lane 2 : Zebrafish whole body homogenate, 17ß-estradiol treated at 10 µg Lane 3 : Zebrafish whole body homogenate at 10 µg Lane 4 : Carp plasma, 17ß-estradiol treated at 10 µl Lane 5 : Carp plasma at 10 µl Lane 6 : Fathead minnow plasma, 17ß-estradiol treated at 10 µl Lane 7 : Fathead minnow plasma at 10 µl Lane 8 : Roach plasma, 17ß-estradiol treated at 10 µl Lane 9 : Roach plasma at 10 µl