製品の概要

  • 製品名
    Anti-Vitellogenin antibody [JE-2A6]
    Vitellogenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [JE-2A6] to Vitellogenin
  • 特異性
    Does not cross-react with vitellogenin from the other cyrinid species carp (Cyprinus carpio), fathead minnow (Pimephales promelas) or roach (Rutilus rutilus).
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Zebrafish
  • 免疫原

    Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    JE-2A6
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/100 - 1/1000.
    Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
    WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • 細胞内局在
      Secreted
    • 別名
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    画像

    • All lanes : Anti-Vitellogenin antibody [JE-2A6] (ab36805) at 1/200 dilution

      Lane 1 : Purified zebrafish vitellogenin at 2 µg
      Lane 2 : Zebrafish whole body homogenate, 17ß-estradiol treated at 10 µg
      Lane 3 : Zebrafish whole body homogenate at 10 µg
      Lane 4 : Carp plasma, 17ß-estradiol treated at 10 µl
      Lane 5 : Carp plasma at 10 µl
      Lane 6 : Fathead minnow plasma, 17ß-estradiol treated at 10 µl
      Lane 7 : Fathead minnow plasma at 10 µl
      Lane 8 : Roach plasma, 17ß-estradiol treated at 10 µl
      Lane 9 : Roach plasma at 10 µl


      Predicted band size : 183 kDa

    プロトコール

    参考文献

    ab36805 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab36805.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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