Anti-Vitellogenin 抗体 [JE-10D4] (ab36804)
Key features and details
- Mouse monoclonal [JE-10D4] to Vitellogenin
- Suitable for: ELISA, WB
- Reacts with: Carp, Zebrafish
- Isotype: IgG1
製品の概要
-
製品名
Anti-Vitellogenin antibody [JE-10D4]
Vitellogenin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [JE-10D4] to Vitellogenin -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Carp, Zebrafish, Brown bullhead, Common roach, Fathead minnow -
免疫原
Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.08% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
JE-10D4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
1/100 - 1/1000.
|
|
WB |
1/100 - 1/1000.
|
特記事項 |
---|
ELISA
1/100 - 1/1000. |
WB
1/100 - 1/1000. |
ターゲット情報
-
関連性
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs). -
細胞内局在
Secreted -
別名
- Vg antibody
- VTG antibody
- VTG1 antibody
画像
-
All lanes : Anti-Vitellogenin antibody [JE-10D4] (ab36804) at 1/500 dilution
Lane 2 : Zebrafish purified vitellogenin at 1 µg
Lane 3 : Zebrafish whole body homogenate (17B-estradiol treated) at 10 µg
Lane 4 : Zebrafish whole body homogenate (control) at 10 µg
Lane 5 : Carp plasma (17B-estradiol treated) at 10 µl
Lane 6 : Carp plasma (control) at 10 µl
Lane 7 : Fathead minno plasma (17B-estradiol treated) at 10 µl
Lane 8 : Fathead minno plasma (control) at 10 µg
Lane 9 : Roach plasma (control) at 10 µg -
ELISA using ab36804 for measurement of vitellogenin in various samples.
ELISA coating: Whole body homogenate (wbh; 10 µg protein/ml) or plasma (pl; diluted 1:1000) from 17β-estradiol treated (E2) or control (C) fsh. Purifed vitellogenin (Vtg; 2 µg/ml).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab36804 は 3 報の論文で使用されています。
- Cozza G et al. The Golgi 'casein kinase' Fam20C is a genuine 'phosvitin kinase' and phosphorylates polyserine stretches devoid of the canonical consensus. FEBS J 285:4674-4683 (2018). PubMed: 30387551
- Li Q et al. FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis. Biosci Rep 35:N/A (2015). PubMed: 26430246
- Fan X et al. Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish. J Biol Chem 285:32946-53 (2010). WB ; Zebrafish . PubMed: 20729204