Vitamin D Receptor overexpression 293T lysate (whole cell) (ab94191)

製品の概要

  • 製品名Vitamin D Receptor overexpression 293T lysate (whole cell)
  • 特記事項ab94191 is a 293T cell transfected lysate in which Human Vitamin D Receptor has been transiently over-expressed using a pCMV-Vitamin D Receptor plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Function: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Disease: Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Vitamin D Receptor overexpression 293T lysate (whole cell) 画像

  • ab94191 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Vitamin D Receptor antibody (ab89626) at 1/500 dilution

    Lane 1 : Vitamin D Receptor overexpression 293T lysate (whole cell) (ab94191)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Vitamin D Receptor overexpression 293T lysate (whole cell) (ab94191) 使用論文

ab94191 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94191.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"