Anti-Vitamin D Receptor 抗体 - ChIP Grade (ab3508)

製品の概要

  • 製品名
    Anti-Vitamin D Receptor antibody - ChIP Grade
    Vitamin D Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vitamin D Receptor - ChIP Grade
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IP, WB, CHIPseq, IHC-P, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    交差が予測される動物種: Cow, Pig, Zebrafish, Saguinus oedipus
  • 免疫原

    Synthetic peptide corresponding to Human Vitamin D Receptor aa 395-413.
    Sequence:

    EEHSKQYRCLSFQPECSMK

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
EMSA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/100. Detects a band of approximately 53 kDa (predicted molecular weight: 48 kDa). 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).
CHIPseq Use at an assay dependent concentration. PubMed: 21846776
IHC-P 1/2000 - 1/4000.
ChIP Use at an assay dependent concentration. PubMed: 17244627Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
  • 関連疾患
    Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 25 dihydroxyvitamin D3 receptor antibody
    • 1 antibody
    • 1,25 dihydroxyvitamin D3 receptor antibody
    • 1,25-@dihydroxyvitamin D3 receptor antibody
    • 25-dihydroxyvitamin D3 receptor antibody
    • Member 1 antibody
    • NR1I1 antibody
    • Nuclear receptor subfamily 1 group I member 1 antibody
    • PPP1R163 antibody
    • Protein phosphatase 1, regulatory subunit 163 antibody
    • VDR antibody
    • VDR_HUMAN antibody
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
    • Vitamin D hormone receptor antibody
    • Vitamin D nuclear receptor variant 1 antibody
    • Vitamin D receptor antibody
    • Vitamin D3 receptor antibody
    see all

画像

  • Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
    Left panel: with primary antibody at 1/2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ab3508 staining the Vitamin D Receptor in mVDR-transfected and untransfected Mouse NIH/3T3 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 1% BSA/5%HS for 30 minutes at 20°C. The sample was incubated with the primary antibody (1/50 in 1% BSA/5% HS in 1xPBS) for 1 hour 30 minutes at 20°C. A Cy3®-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary.

    See Abreview

  • ab3508 at a 1/2000 dilution staining Vitamin D Receptor in whole Rat embryo tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. Samples fixed in 4% PFA prior to cutting at 30µm thickness. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.

    See Abreview

参考文献

This product has been referenced in:
  • Houari S  et al. Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors. Front Physiol 7:503 (2016). IHC-P ; Rat . Read more (PubMed: 27853434) »
  • Abreu-Delgado Y  et al. Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease. World J Gastroenterol 22:3581-91 (2016). Read more (PubMed: 27053850) »

See all 24 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH/3T3, untransfected, mVDR-transfected)
Specification
NIH/3T3, untransfected, mVDR-transfected
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
1% BSA, 5% HS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. A Uhmann

Verified customer

投稿 Dec 03 2009

Thank you for contacting us.

Since this is a whole antiserum antibody, the concentration has not been determined. Typically the total protein concentration for these antibodies can range from 1 to 10 mg/ml (you could split the difference and...

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Thank you very much for contacting us with your enquiry.

Ab3508 is an unpurified whole serum antibody, and as there are other proteins in the serum the concentration of specific IgG can not be measured. This antibody has been used in ChIP in ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Whole embryo)
Specification
Whole embryo
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (NIH/3T3, untransfected, VDR-transfected)
Loading amount
10 µg
Specification
NIH/3T3, untransfected, VDR-transfected
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Miss. A Uhmann

Verified customer

投稿 Dec 01 2009

Thank you for your enquiry. The resources I have available indicate that 1 ul of ab3508 should be a sufficient amount for immunoprecipitation assays. I would recommend using a minimum of 100 ug of sample. I hope this information helps, please do...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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