Anti-Vitamin D Binding protein 抗体 (ab65636)
製品の概要
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製品名Anti-Vitamin D Binding protein antibody
Vitamin D Binding protein 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Vitamin D Binding protein
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由来種Rabbit
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アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Vitamin D Binding Protein.
(Peptide available as ab71146.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: human liver and human ovary IHC-P: Human kidney FFPE tissue sections.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab65636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
ターゲット情報
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機能Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
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配列類似性Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
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別名
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody (ab65636)IHC image of Vitamin D Binding Protein staining in human kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65636, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
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Western blot - Anti-Vitamin D Binding protein antibody (ab65636)All lanes : Anti-Vitamin D Binding protein antibody (ab65636) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute -
Western blot - Anti-Vitamin D Binding protein antibody (ab65636) -
Immunocytochemistry/ Immunofluorescence - Anti-Vitamin D Binding protein antibody (ab65636)ICC/IF image of ab65636 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65636, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Ackermann AM et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016). Human . Read more (PubMed: 26977395) »
- Taniuchi K et al. RUVBL1 directly binds actin filaments and induces formation of cell protrusions to promote pancreatic cancer cell invasion. Int J Oncol 44:1945-54 (2014). Read more (PubMed: 24728183) »
レビューと Q&A
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