製品の概要

  • 製品名
    Anti-Visfatin antibody [4D5]
    Visfatin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4D5] to Visfatin
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human) purified from E. coli

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • 組織特異性
    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • パスウェイ
    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • 配列類似性
    Belongs to the NAPRTase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

画像



  • Predicted band size : 56 kDa

    (Top) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : 293T cell lysate (30ug)

    (Bottom) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : SW480 cell lysate (40ug)

  • ICC/IF analysis of Visfatin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Visfatin antibody (1:100) with goat anti-mouse IgG-Alexa fluor® 488 conjugate (Green).

プロトコール

参考文献

This product has been referenced in:
  • James EL  et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). Read more (PubMed: 27924925) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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