製品の概要

  • 製品名Anti-Visfatin antibody [4D5]
    Visfatin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4D5] to Visfatin
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human) purified from E. coli

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • 組織特異性Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • パスウェイCofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • 配列類似性Belongs to the NAPRTase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
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    • Nampt antibody
    • NAMPT_HUMAN antibody
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    • PBEF antibody
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    • Pre-B cell-enhancing factor antibody
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    see all

Anti-Visfatin antibody [4D5] 画像



  • Predicted band size : 56 kDa

    (Top) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : 293T cell lysate (30ug)

    (Bottom) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : SW480 cell lysate (40ug)

  • ICC/IF analysis of Visfatin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Visfatin antibody (1:100) with goat anti-mouse IgG-Alexa fluor® 488 conjugate (Green).

Anti-Visfatin antibody [4D5] (ab71505) 使用論文

ab71505 has not yet been referenced specifically in any publications.

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