製品の概要

  • 製品名Anti-Viperin antibody
    Viperin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Viperin
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse, Dog, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Viperin.

    (Peptide available as ab73863.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Lysates: Mouse Spinal Cord Tissue, SK N SH Whole Cell, Rat Spinal Cord Tissue, Human Brain Tissue, Mouse Brain Tissue It also gave a positive result in U87-MG cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

ターゲット情報

  • 機能Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus.
  • 配列類似性Belongs to the RSAD2 family.
  • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus. Probably associates with the cytosolic side of the endoplasmic reticulum. Infection with human cytomegalovirus (HCMV) causes relocation to the Golgi apparatus and to cytoplasmic vacuoles which also contain HCMV proteins glycoprotein B and pp28.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2510004L01Rik antibody
    • cig 33 antibody
    • CIG 5 antibody
    • cig-33 antibody
    • CIG-5 antibody
    • CIG33 antibody
    • CIG5 antibody
    • Cytomegalovirus induced gene 5 protein antibody
    • Cytomegalovirus-induced gene 5 protein antibody
    • endoplasmic reticulum-associated antibody
    • interferon-inducible antibody
    • Radical S adenosyl methionine domain containing 2 antibody
    • Radical S-adenosyl methionine domain-containing protein 2 antibody
    • RSAD 2 antibody
    • Rsad2 antibody
    • RSAD2_HUMAN antibody
    • RSDA-2 antibody
    • VIG 1 antibody
    • vig1 antibody
    • Viperin antibody
    • Virus inhibitory protein antibody
    • virus inhibitory protein endoplasmic reticulum associated interferon inducible antibody
    see all

Anti-Viperin antibody 画像

  • All lanes : Anti-Viperin antibody (ab73864) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : Spinal Cord (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 30 seconds
  • ICC/IF image of ab78364 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Viperin antibody (ab73864) 使用論文

This product has been referenced in:
  • Mangeat B  et al. Influenza virus partially counteracts restriction imposed by tetherin/BST-2. J Biol Chem 287:22015-29 (2012). WB . Read more (PubMed: 22493439) »
  • Kroeker AL  et al. Response of primary human airway epithelial cells to influenza infection: a quantitative proteomic study. J Proteome Res 11:4132-46 (2012). WB . Read more (PubMed: 22694362) »

See all 3 Publications for this product

Product Wall

DISCOUNT CODE: XXXX
Expiration date: 03-08-2013
Value: €380

I am very pleased to hear you would like to accept our offer and test ab73864 in Porcine. This code will give you €380 off your next order before the expiration date...

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Thank you for contacting us.
The immunogen is 84% homologous to Porcine sequence. Though this antibody has not been tested but it is more likely to work.
In case you are interested in trying this antibody then I would encourage you to partici...

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Vielen Dank für Ihre Anfrage.

Es freut mich zu hören, dass ab73864 im Western blot gut funktioniert.

Wir haben diesen Antikörper bisher noch nicht in der Immunhistochemie (IHC) getestet, und wissen daher leider nicht,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"