製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VIP
  • 特異性
    ab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
  • アプリケーション
    適用あり: IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Sheep, Dog, Human, Pig
  • 免疫原

    Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.

  • ポジティブ・コントロール
    • Human, rat and porcine colon and spinal cord.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
IHC-P
  • 追加情報
    IHC-Fr: 1/400 - 1/1600.
    IHC-P: 1/400 - 1/1600.
    Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
    Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
    using overnight incubation and indirect immunofluorescence procedure.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • ab22736 staining VIP in jejunum tissue section of pig by Immunohistochemistry (frozen sections). Tissue underwent fixation in paraformaldehyde and incubated with primary antibody, diluted 1/200 for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit, dilution 1/400 was used as the secondary.

      See Abreview

    • ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary. 

    プロトコール

    参考文献

    This product has been referenced in:
    • Hallett PJ  et al. a-Synuclein overexpressing transgenic mice show internal organ pathology and autonomic deficits. Neurobiol Dis 47:258-67 (2012). Read more (PubMed: 22549133) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (jejunum)
    Specification
    jejunum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    投稿 Nov 06 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Sheep Tissue sections (abomasum)
    Specification
    abomasum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    投稿 Oct 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (duodenum)
    Specification
    duodenum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    投稿 Jun 22 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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