製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to Vinexin
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Vinexin protein (Human)

  • ポジティブ・コントロール
    • Vinexin transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 75 kDa).

ターゲット情報

  • 関連性
    Vinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
  • 細胞内局在
    Localized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
  • 参照データベース
  • 別名
    • SCAM 1 antibody
    • SCAM1 antibody
    • SH3 containing adapter molecule 1 antibody
    • SH3 domain containing adapter molecule 1 antibody
    • SH3D4 antibody
    • Sorbin and SH3 domain containing 3 antibody
    • Sorbin and SH3 domain containing protein 3 antibody
    • Sorbs3 antibody
    • Vinexin alpha antibody
    • Vinexin antibody
    • Vinexin beta (SH3 containing adaptor molecule 1) antibody
    see all

画像

  • All lanes : Anti-Vinexin antibody (ab68222) at 1/500 dilution

    Lane 1 : Vinexin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 75 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab68222 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

投稿 Nov 16 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

投稿 Nov 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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