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Full length native protein (purified) corresponding to Human Vinculin.
Our Abpromise guarantee covers the use of ab18058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200. See Abreview.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 130 kDa.
130kDa (vinculin) and 150kDa (meta-vinculin)
|IHC-P||1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab18058 staining mouse 3T3 cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C, ab6785, diluted 1/100, was used as the secondary antibody.
Lanes: 1, 4, 7 - Hamster Lung; 2, 5, 8 - K562; 3, 6, 9 - CHO
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"