Anti-Vinculin 抗体 (ab91459)
Key features and details
- Rabbit polyclonal to Vinculin
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Vinculin antibody
Vinculin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Vinculin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus -
免疫原
Synthetic peptide corresponding to Human Vinculin aa 1000 to the C-terminus (C terminal).
Database link: P18206-2 -
ポジティブ・コントロール
- HeLa, 293T or NIH3T3 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91459の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
ターゲット情報
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機能
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. -
組織特異性
Metavinculin is muscle-specific. -
関連疾患
Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. -
配列類似性
Belongs to the vinculin/alpha-catenin family. -
ドメイン
Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly. -
翻訳後修飾
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
Aceylated; mainly by myristic acid but also small amount of palmitic acid. -
細胞内局在
Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. - Information by UniProt
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参照データベース
- Entrez Gene: 396422 Chicken
- Entrez Gene: 7414 Human
- Entrez Gene: 22330 Mouse
- Entrez Gene: 396974 Pig
- Entrez Gene: 305679 Rat
- Omim: 193065 Human
- SwissProt: P12003 Chicken
- SwissProt: P18206 Human
see all -
別名
- CMD1W antibody
- CMH15 antibody
- Epididymis luminal protein 114 antibody
see all
画像
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All lanes : Anti-Vinculin antibody (ab91459) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Vinculin with ab91459 at 1/1000 (1µg/ml). Detection: DAB.
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SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91459.
20% of the immunoprecipitate was loaded per lane, and probed with ab91459 at 1µg/ml (lane 1) or a control IgG (lane2).
Detection: chemoluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab91459 は 10 報の論文で使用されています。
- Zheng Z et al. Adeno-associated viral delivery of engineered tRNA-enzyme pairs into nonsense mutation mouse models. STAR Protoc 4:101950 (2022). PubMed: 36527714
- Didychuk AL et al. A pentameric protein ring with novel architecture is required for herpesviral packaging. Elife 10:N/A (2021). PubMed: 33554858
- McLaren SBP & Steventon BJ Anterior expansion and posterior addition to the notochord mechanically coordinate zebrafish embryo axis elongation. Development 148:N/A (2021). PubMed: 34086031
- Greco SJ et al. Restoration of aged hematopoietic cells by their young counterparts through instructive microvesicles release. Aging (Albany NY) 13:23981-24016 (2021). PubMed: 34762598
- Missios P et al. LIN28B alters ribosomal dynamics to promote metastasis in MYCN-driven malignancy. J Clin Invest 131:N/A (2021). PubMed: 34779407
- Blanco AM et al. In vitro insulin treatment reverses changes elicited by nutrients in cellular metabolic processes that regulate food intake in fish. J Exp Biol 223:N/A (2020). PubMed: 32179544
- Chen Y et al. Remodeling of the H3 nucleosomal landscape during mouse aging. Transl Med Aging 4:22-31 (2020). PubMed: 32462102
- Hirth S et al. Paxillin and Focal Adhesion Kinase (FAK) Regulate Cardiac Contractility in the Zebrafish Heart. PLoS One 11:e0150323 (2016). WB, IF . PubMed: 26954676
- Simon NC & Barbieri JT Bacillus cereus Certhrax ADP-ribosylates vinculin to disrupt focal adhesion complexes and cell adhesion. J Biol Chem 289:10650-9 (2014). WB ; Human . PubMed: 24573681
- Thompson WR et al. Mechanically activated Fyn utilizes mTORC2 to regulate RhoA and adipogenesis in mesenchymal stem cells. Stem Cells 31:2528-37 (2013). PubMed: 23836527