製品の概要

  • 製品名Anti-Vinculin antibody
    Vinculin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vinculin
  • アプリケーション適用あり: IHC-P, ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide (Human) from around the phosphorylation site of tyrosine 821 (SGYPRI)

  • ポジティブ・コントロール
    • Human breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ELISA 1/10000.
WB 1/500 - 1/1000.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
  • 組織特異性Metavinculin is muscle-specific.
  • 関連疾患Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • 配列類似性Belongs to the vinculin/alpha-catenin family.
  • ドメインExists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
    The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly.
  • 翻訳後修飾Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
    Aceylated; mainly by myristic acid but also small amount of palmitic acid.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CMD1W antibody
    • CMH15 antibody
    • Epididymis luminal protein 114 antibody
    • HEL114 antibody
    • Metavinculin antibody
    • MV antibody
    • MVCL antibody
    • OTTHUMP00000019861 antibody
    • OTTHUMP00000019862 antibody
    • VCL antibody
    • VINC antibody
    • VINC_HUMAN antibody
    • Vinculin antibody
    see all

Anti-Vinculin antibody 画像

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab61186 at 1/50 dilution. Samples were treated -/+ immunising peptide.
  • All lanes : Anti-Vinculin antibody (ab61186)

    Lane 1 : Hela, treated with forskolin 40nM 30'
    Lane 2 : Synthesized Peptide
  • ab61186 staining A549 cells, image on right treated with synthesized peptide.

Anti-Vinculin antibody (ab61186) 使用論文

This product has been referenced in:
  • Baeyens N  et al. Identification and functional implication of a Rho kinase-dependent moesin-EBP50 interaction in noradrenaline-stimulated artery. Am J Physiol Cell Physiol 299:C1530-40 (2010). WB ; Rat . Read more (PubMed: 20926777) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
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Abcam user community

Verified customer

投稿 Nov 16 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line ( cervical cancer))
Specification HeLa cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

投稿 Nov 16 2011

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

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Thank you for contacting us and for reporting the problem you experienced with the antibody ab61186. I am sorry that the product you received did not perform as stated expected. We have recently updated the datasheet and the antibody should ind...

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Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Neonatal Ventricular Myocyte)
Specification Neonatal Ventricular Myocyte
Fixative Paraformaldehyde
Permeabilization Yes - Triton X 100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Natasha Mehta

Verified customer

投稿 May 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"