Anti-Vimentin (phospho S83) 抗体 (ab79189)

製品の概要

  • 製品名Anti-Vimentin (phospho S83) antibody
    Vimentin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vimentin (phospho S83)
  • 特異性ab79189 detects endogenous levels of Vimentin only when phosphorylated at serine 83.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).

  • ポジティブ・コントロール
    • 293 cytoplasmic lysate treated with paclitaxel (1 µM, 24 hours); human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 関連疾患Cataract 30
  • 配列類似性Belongs to the intermediate filament family.
  • ドメインThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻訳後修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Vimentin is found in connective tissue and in the cytoskeleton.
  • 別名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin (phospho S83) antibody 画像

  • All lanes : Anti-Vimentin (phospho S83) antibody (ab79189) at 1/500 dilution

    Lane 1 : 293 cells treated with paclitaxel (1 µM, 24 hours)
    Lane 2 : 293 cells treated with paclitaxel (1 µM, 24 hours) with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab79189, at a 1/50 dilution, staining Vimentin in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    Left image: no immunizing peptide.
    Right image: with immunizing phospho peptide.

Anti-Vimentin (phospho S83) antibody (ab79189) 使用論文

ab79189 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"