製品の概要

  • 製品名Anti-Vimentin (phospho S82) antibody [EP1071Y]
    Vimentin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
  • 特異性ab52943 only detects Vimentin phosphorylated at Serine 82.
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic acetylated peptide corresponding to residues surrounding Ser82 of Vimentin

  • ポジティブ・コントロール
    • AP treated and untreated HeLa + Calyculin A cell lysate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

    Is unsuitable for Flow Cyt, IHC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
    • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
    • 関連疾患Cataract 30
    • 配列類似性Belongs to the intermediate filament family.
    • ドメインThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    • 翻訳後修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 製品の状態Vimentin is found in connective tissue and in the cytoskeleton.
    • 別名
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin (phospho S82) antibody [EP1071Y] 画像

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: Vimentin (pS82) phospho peptide

      Lane 2 sample: Vimentin non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution

      Lane 1 : HeLa cell lysate AP treated
      Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa

    Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) 使用論文

    This product has been referenced in:
    • Goto H  et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). Read more (PubMed: 26795468) »
    • Haolong C  et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24073199) »

    See all 3 Publications for this product

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