製品の概要

  • 製品名Anti-Vimentin (phospho S71) antibody [TM71]
    Vimentin 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [TM71] to Vimentin (phospho S71)
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide (SAVRLRSSVPGV), corresponding to amino acids 66-77 of Human Vimentin phosphorylated at Ser71.

  • ポジティブ・コントロール
    • U251 cells and cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 関連疾患Cataract 30
  • 配列類似性Belongs to the intermediate filament family.
  • ドメインThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻訳後修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Vimentin is found in connective tissue and in the cytoskeleton.
  • 別名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin (phospho S71) antibody [TM71] 画像

  • IHC image of Vimentin (phospho S71) staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115189, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Vimentin (phospho S71) antibody [TM71] (ab115189) at 1 µg/ml + U251 cell lysate
    Developed using the ECL technique

    Predicted band size : 54 kDa
  • ab115189 at 1µg/ml staining Vimentin in 4% PFA fixed U251 cells by Immunofluorescence.

Anti-Vimentin (phospho S71) antibody [TM71] (ab115189) 使用論文

This product has been referenced in:
  • Chi C  et al. In vitro morphology, viability and cytokine secretion of uterine telocyte-activated mouse peritoneal macrophages. J Cell Mol Med 19:2741-50 (2015). ICC/IF ; Mouse . Read more (PubMed: 26471943) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab115189.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"