製品の概要

  • 製品名Anti-Vimentin (phospho S56) antibody
    Vimentin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vimentin (phospho S56)
  • 特異性Detects Vimentin only when phosphorylated at serine 56.
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).

  • ポジティブ・コントロール
    • Extracts from A549 cells treated with Nocodazole (1ug/ml, 16hours), human breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 機能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 関連疾患Cataract 30
  • 配列類似性Belongs to the intermediate filament family.
  • ドメインThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻訳後修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Vimentin is found in connective tissue and in the cytoskeleton.
  • 別名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin (phospho S56) antibody 画像

  • All lanes : Anti-Vimentin (phospho S56) antibody (ab61802) at 1/500 dilution

    Lane 1 : extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours)
    Lane 2 : extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours) with immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Ab61802 at 1/50 dilution staining human breast carcinoma tissue without (left) and with (right) immunizing peptide; paraffin embedded.

Anti-Vimentin (phospho S56) antibody (ab61802) 使用論文

This product has been referenced in:
  • Thaiparambil JT  et al. Withaferin A inhibits breast cancer invasion and metastasis at sub-cytotoxic doses by inducing vimentin disassembly and serine 56 phosphorylation. Int J Cancer 129:2744-55 (2011). WB . Read more (PubMed: 21538350) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab61802.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"