製品の概要

  • 製品名Anti-Vimentin antibody [Vim 3B4]
    Vimentin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Vim 3B4] to Vimentin
  • 特異性Ab28028 recognises Vimentin
  • アプリケーション適用あり: WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis
  • 免疫原

    Full length native protein (purified): Vimentin isolated from bovine eye lens.

  • エピトープAb28028 reacts with an epitope located in the coil 2 part of the vimentin rod domain.
  • ポジティブ・コントロール

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/200. Predicted molecular weight: 54 kDa.
IHC-Fr 1/100 - 1/200. The antibody can be used for labeling acetone fixed, frozen sections.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 関連疾患Cataract 30
  • 配列類似性Belongs to the intermediate filament family.
  • ドメインThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻訳後修飾Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Vimentin is found in connective tissue and in the cytoskeleton.
  • 別名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody [Vim 3B4] 画像

  • ab28028 staining of tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab28028 staining of tonsil by Immunohistochemistry(Formalin-fixed paraffin-embedded sections)

  •  ab28028 at 1/200 dilution staining vimentin in human lung cancer cells. The Vimentin staining is shown in red. The cells were counterstained with DAPI. Please see accompanying abreview for additional information.

    See Abreview

  • ab28028 staining Vimentin in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton x100. Samples were incubated with primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor®488-conjugated donkey monoclonal to mouse IgG (ab150105) was used at dilution at 1/500 as secondary antibody.

    See Abreview

Anti-Vimentin antibody [Vim 3B4] (ab28028) 使用論文

This product has been referenced in:
  • Cui Y  et al. Asparaginyl endopeptidase promotes the invasion and metastasis of gastric cancer through modulating epithelial-to-mesenchymal transition and analysis of their phosphorylation signaling pathways. Oncotarget 7:34356-70 (2016). Human . Read more (PubMed: 27102302) »
  • Buchholz M  et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS One 10:e0122946 (2015). ICC . Read more (PubMed: 25849100) »

See all 12 Publications for this product

Product Wall

Merci de nous avoir contactés.

L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration ...

Read More

Thank you for contacting us.

We have 4, FITC conjugated anti Glycophorin A antibodies.

http://www.abcam.com/Glycophorin-A-antibody-YTH89-1-FITC-ab28082.html
http://www.abcam.com/Glycophorin-A-antibody-FITC-ab112201.html
http:/...

Read More

Thank you for your inquiry.

For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

Read More

Thank you for contacting us and your interest in our products.


We have several antibodies directed against epithelial membrane antigen (also known as MUC1), however only one has been teated in all the applications you wish to use it for...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - 1% TX-100
Username

Abcam user community

Verified customer

投稿 Oct 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung cancer)
Specification Lung cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Da Zhu

Verified customer

投稿 Mar 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"