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Recombinant human vimentin.
Conjugated with R-Phycoerythrin (PE) under optimum conditions.
Our Abpromise guarantee covers the use of ab49918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use a concentration of 10 µg/ml.
Working concentrations should be determined by the investigator.
ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Flow cytometric analysis of Human stromal endometrial somatic stem cells, staining Vimentin with ab49918.
Gating Strategy: Live cells